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For educational use only — The physician’s written order and the unit protocol always govern
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Dialysis Nursing Reference Card  ·  Maintenance Hemodialysis

N7 — AVF/AVG Assessment and Cannulation

Look, listen, feel, and test before every needle. The access exam is the surveillance.
Andrew Bland, MD, FACP, FAAP Reviewed September 2026 · v1.0 draft Prints on one sheet, front and back
N7 — AVF/AVG Assessment and Cannulation FRONT
Front · at the chair

When: before every cannulation, and at needle removal.

Do this

  1. Wash and look. The patient washes the access arm before cannulation. Look for redness, swelling, drainage, skin breakdown, thin or shiny skin over an aneurysm, a cool or discolored hand, and neck or chest collaterals [5].
  2. Listen. Normal: low-pitched, continuous bruit. Abnormal: high-pitched, systolic-only, or absent.
  3. Feel. Normal: soft, compressible vessel with a thrill. Abnormal: hard pulsatile segment, weak thrill, “mushy” graft, or no thrill.
  4. Test. Raise the arm — a fistula should collapse. Occlude above the anastomosis — the pulse should augment.
  5. Confirm flow direction before placing needles; use ultrasound for a new or difficult access if trained.
  6. Rope ladder: rotate both sites along the full length of the access. No area puncture [5].
  7. Needles: gauge matched to the prescribed blood flow per your unit protocol. Venous needle always antegrade; avoid a retrograde, bevel-down arterial needle [6].
  8. First cannulations of a new fistula go to the most skilled cannulator.
  9. After dialysis: firm pressure on the puncture site — enough to stop bleeding, not enough to lose the thrill.

Call the nephrologist when

  • Same day: no thrill and no bruit; a rapidly expanding hematoma; a cold, pale, painful, or weak hand; bleeding you cannot control; thin, shiny, ulcerated, or scabbed skin over an aneurysm.
  • Before the next session: pus, spreading redness, or fever — cultures before antibiotics.
  • At rounds: new difficulty cannulating, clots aspirated, prescribed blood flow not reached, needle sites bleeding longer than usual for 3 sessions in a row, an unexplained Kt/V drop of more than 0.2, or arm, face, or breast swelling [5].

Don’t

  • Don’t cannulate a fistula within 14 days of creation [3], or before the access team has cleared it.
  • Don’t buttonhole a graft — ever. Don’t start a buttonhole without an order and an indication [2].
  • Don’t needle the top of an aneurysm.
  • Don’t allow BP cuffs, IVs, or blood draws on the access arm (per your unit protocol); flag any PICC request to the nephrologist.
  • Don’t press on the vessel downstream of a bleeding point.
N7 — AVF/AVG Assessment and Cannulation BACK
Back · why, and the evidence

Why

  1. Physical exam by trained staff agreed with angiography in about 89% of outflow and 80% of inflow stenoses [1].
  2. Buttonhole cannulation carried 2.6 times the access-related bloodstream infection risk of rope ladder in national CDC data [2]; S. aureus bacteremia was 13% versus 0% at 1 year in a randomized trial [7].
  3. Cannulating a fistula within 14 days of creation was associated with 2.1-fold higher risk of fistula failure [3].
  4. A major infiltration added a median of 97 days on a catheter, and 26% of infiltrated fistulas clotted [4].
  5. KDOQI 2019: examine the access before every cannulation; surveillance numbers are supplementary — act on clinical findings [5].

Go deeper

References

  1. Asif A, et al. Accuracy of physical examination in the detection of arteriovenous fistula stenosis. Clin J Am Soc Nephrol. 2007;2:1191-1194. PubMed 17928468
  2. Lyman M, et al. Risk of vascular access infection associated with buttonhole cannulation of fistulas (NHSN). Am J Kidney Dis. 2020;76:82-89. PubMed 32151430
  3. Rayner HC, et al. Creation, cannulation and survival of arteriovenous fistulae: data from DOPPS. Kidney Int. 2003;63:323-330. PubMed 12472799
  4. Lee T, Barker J, Allon M. Needle infiltration of arteriovenous fistulae in hemodialysis: risk factors and consequences. Am J Kidney Dis. 2006;47:1020-1026. PubMed 16731297
  5. Lok CE, et al. KDOQI clinical practice guideline for vascular access: 2019 update. Am J Kidney Dis. 2020;75(4 Suppl 2):S1-S164. PubMed 32778223
  6. Parisotto MT, et al. Cannulation technique influences arteriovenous fistula and graft survival. Kidney Int. 2014;86:790-797. PubMed 24717298
  7. MacRae JM, et al. Arteriovenous fistula survival and needling technique: long-term results from a randomized buttonhole trial. Am J Kidney Dis. 2014;63:636-642. PubMed 24239019

Reviewed by Andrew Bland, MD, FACP, FAAP · v1.0 draft 2026-09-26. For education; the physician’s written order and the unit protocol always govern.